Journal: The Journal of Biological Chemistry

Article Title: Small molecule intervention of actin-binding protein profilin1 reduces tumor angiogenesis in renal cell carcinoma

doi: 10.1016/j.jbc.2025.111033

Figure Lengend Snippet: Demonstration of C74’s ability to diminish tumor angiogenesis . A , chemical structure of C74. B - C , representative images ( panel B ; 4 × magnification) and quantification ( panel C ; based on 10 × field images) of CD31 + cells in subcutaneously established RENCA tumors subjected to daily direct intratumoral injection for 19 days with either C74 or equivalent amount of DMSO control in BALB/c mice (two-tailed Student’s t test, ∗∗, p < 0.01; n = 5 mice/group; the scale bar represents 200 μm). DMSO, dimethyl sulfoxide.

Article Snippet: RENCA renal cell carcinoma (RCC) cells (American Type Culture Collection [ATCC], CRL-2947) were cultured in RPMI supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin.

Techniques: Injection, Control, Two Tailed Test

Journal: The Journal of Biological Chemistry

Article Title: Small molecule intervention of actin-binding protein profilin1 reduces tumor angiogenesis in renal cell carcinoma

doi: 10.1016/j.jbc.2025.111033

Figure Lengend Snippet: Feasibility of ultrasound-guided tumor-localized delivery of Pfn1 inhibitor to achieve antiangiogenic and therapeutic benefit. A , experimental schema of tumor microbubble studies (MB–microbubbles). B , ultrasound images ( panel B ) showing MB infiltration into tumor vasculature ( white arrows ), followed by UTMD and no contrast remaining in the tumor space. C – J , results of C74-MB ( panels C through F ) and UP6-MB ( panels G – J ) tumor model studies. Images of subcutaneous RENCA tumors subjected to UTMD of indicated MBs ( panels C and G ) with end point tumor burden comparison between the control and experimental treatment groups ( panels D and H ). Representative H&E images of tumor histosections ( panels E and I; white arrows indicate functional tumor-associated blood vessels with presence of red blood cells) and the associated quantification ( panels F and I ) of the average number of tumor-associated blood vessels per 20X field of observation between the two treatment groups (Mann-Whitney test, ∗: p < 0.05; ∗∗ p < 0.01, ∗∗∗: p < 0.001; data pooled from 8-9 mice per group; the scale bar represents 50 μm). Pfn1, profilin1; UTMD, ultrasound-targeted microbubble destruction.

Article Snippet: RENCA renal cell carcinoma (RCC) cells (American Type Culture Collection [ATCC], CRL-2947) were cultured in RPMI supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin.

Techniques: Comparison, Control, Functional Assay, MANN-WHITNEY

Journal: Cell Reports Medicine

Article Title: Intestinal Lachnospiraceae bacterium -derived propionate inhibits the progression of clear cell renal cell carcinoma

doi: 10.1016/j.xcrm.2025.102410

Figure Lengend Snippet: L. bacterium protects against ccRCC tumorigenesis through its supernatant (A) Experimental design: After gut microbiota depletion with ATB, RENCA-bearing mice were gavaged with L. bacterium or P. copri 5 times/week ( n = 6/group). (B and C) Tumor growth kinetics (B) and tumor weights (C) on day 24 in mice receiving L. bacterium or P. copri ( n = 6/group). (D) 16S rRNA sequencing of fecal samples from control and L. bacterium -treated mice on days 12 and 24. (E) Experimental design: RENCA-bearing mice pretreated with ATB were gavaged with L. bacterium with or without amoxicillin in drinking water ( n = 9/group). (F and G) Tumor growth curves (F) and tumor weights (G) on day 24 in the indicated groups ( n = 9/group). (H–J) Effects of L. bacterium SN on 786-O and Caki-1 cells: colony formation (H), cell viability via CCK8 assay (I), and wound healing assay (J) with 100× and 10× dilutions of SN. Scale bars: 200 μm. (K) Experimental design: ATB-pretreated RENCA-bearing mice were gavaged with L. bacterium , heat-inactivated L. bacterium , or SN ( n = 9/group). (L and M) Tumor weights in BALB/c (L) and BALB/c-nude mice (M) on day 24 ( n = 9–10/group). Data are shown as mean ± SEM. Statistical significance was determined by two-way ANOVA (B, F, and I), unpaired two-tailed t test (C, G, L, and M), and paired two-tailed t test (J). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. See also and .

Article Snippet: The human cell lines 786-O, Caki-1, and 293T, as well as the murine renal carcinoma cell line RENCA, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Sequencing, Control, CCK-8 Assay, Wound Healing Assay, Two Tailed Test